Journal: PLOS One
Article Title: Prognostic value of cGAS-STING-IRF3 signaling in cholangiocarcinoma patients
doi: 10.1371/journal.pone.0342756
Figure Lengend Snippet: A . (A-C) FFPE from a CCA patients of H&E staining, 20X, scale bar = 100 µm of Well, Moderate and Poorly differentiated papillary subtype. (D-F) Weak (1+), Moderate (2+), or Strong (3+) of IRF3 expression patterns. Represents staining, 20X, scale bar = 100 µm. B . (A-C) FFPE from a CCA patients of H&E staining, 20X, scale bar = 100 µm of Well, Moderate and Poorly differentiated tubular subtype. (D-F) Weak (1+), Moderate (2+), or Strong (3+) of IRF3 expression patterns. Represents staining, 20X, scale bar = 100 µm.
Article Snippet: Primary antibodies used included a rabbit polyclonal Gamma H2A.X antibody (phosphor S139) (γH2AFX; dilution 1:400, ab11174; Abcam), rabbit polyclonal anti-human c-GAS antibody (dilution 1:200, Proteintech, US), rabbit polyclonal anti-human STING antibody (dilution 1:3000, Proteintech, US), rabbit polyclonal anti-human IRF3 antibody (dilution 1:800, Proteintech, US), and rabbit polyclonal anti-human IFN alpha antibody (dilution 1:50, Proteintech, US).
Techniques: Staining, Expressing